Oxygen Binding–Dissociation Analyzer

The Bloodox-2019 Oxygen Binding–Dissociation Analyzer continuously records hemoglobin oxygen binding and release across a range of oxygen partial pressures. It generates oxygen-binding and oxygen-dissociation curves and calculates P50, the Hill coefficient, and other parameters for assessing oxygen affinity and oxygen transport characteristics. Suitable for whole blood, red blood cell lysates, hemoglobin solutions, hemoglobin-based oxygen carriers, and related samples.

1. Key Features

1. Direct PO₂ Measurement with a Clark Oxygen Electrode

A Clark oxygen electrode continuously measures sample oxygen partial pressure (PO₂).

This established electrochemical method responds directly to changes in the oxygen partial pressure of the sample.

Bloodox-2019 combines Clark-electrode PO₂ measurement with dual-wavelength spectrophotometry to record, in real time:

Changes in oxygen partial pressure + changes in hemoglobin oxygenation

The result is a direct relationship between PO₂ and hemoglobin oxygenation status.

2. Dynamic Oxygen Binding and Release Profiles

The system continuously tracks hemoglobin responses as PO₂ changes:

Oxygen binding → higher oxygen saturation

Oxygen release → lower oxygen saturation

Complete oxygen-binding and oxygen-dissociation curves are generated.

These curves provide an intuitive view of hemoglobin oxygen binding and release at different PO₂ levels, enabling comparison between samples and test conditions.

3. Microliter-Scale Sample Requirement

Each test requires approximately 3–6 mg of hemoglobin.

For whole blood, the typical sample volume is approximately 30–50 µL—ideal for limited or valuable samples and multi-group studies.

4. Compatible Samples

Whole blood · Red blood cell lysates
Hemoglobin solutions
Hemoglobin-based oxygen carriers
Other samples requiring assessment of hemoglobin oxygen binding and release

5. Integrated Control for Reproducible Testing

Bloodox-2019 integrates gas delivery, temperature control, PO₂ monitoring, sample handling, and data acquisition for controlled oxygen-binding and oxygen-dissociation testing.

(1) Precision Gas-Flow Control

Air and nitrogen flow can be set from 0 to 20 mL/min to control oxygenation and deoxygenation.

Adjustable gas flow provides precise control over changes in the sample oxygen environment.

(2) Precision Temperature Control

A microprocessor-controlled system continuously monitors and regulates the sample-chamber temperature.

The measurement temperature is maintained at 37 ± 0.1 °C, helping minimize the effect of temperature variation on oxygen-affinity results.

(3) Centralized Touchscreen Control

The touchscreen interface provides control of:

Air / nitrogen selection
Gas-flow settings
Oxygenation / deoxygenation
Sample aspiration
Sample discharge
Real-time PO₂ monitoring
Real-time temperature monitoring
Centralized control reduces repetitive manual steps and supports a more consistent workflow.

2. Measurement Principle

Deoxyhemoglobin and oxyhemoglobin have distinct optical absorption characteristics. Bloodox-2019 uses dual-wavelength spectrophotometry to monitor hemoglobin oxygenation while a Clark oxygen electrode measures sample PO₂ in real time. Air or nitrogen drives oxygenation or deoxygenation. Continuous acquisition of PO₂ and hemoglobin oxygenation data establishes their dynamic relationship and produces an oxygen-binding or oxygen-dissociation curve.

3. Sample Chamber

The sample chamber integrates: Clark oxygen electrode | Temperature sensor | Magnetic stirring system

PO₂ and temperature are monitored simultaneously, while magnetic stirring keeps the sample homogeneous.

Connected to the gas, aspiration, and discharge lines, the chamber supports touchscreen-controlled:

Automatic sample aspiration
Air / nitrogen delivery
Sample discharge
Minimizes manual handling during testing.

4. Data Analysis

1. Oxygen-Binding and Oxygen-Dissociation Curves

The software displays hemoglobin oxygen binding or release in real time as PO₂ changes. Processing options include:

Raw Curve
Translation
Smooth
LineFit
Zero-Completion
Raw-data export

2. P50

The oxygen partial pressure at which hemoglobin reaches 50% oxygen saturation. P50 is a key indicator of hemoglobin oxygen affinity.

In general:

Higher P50 Lower oxygen affinity and a greater tendency to release oxygen

Lower P50 Higher oxygen affinity and a greater tendency to bind oxygen

3. Hill Coefficient

The Hill equation is used to assess cooperativity in hemoglobin–oxygen binding and to compare oxygen-binding characteristics across samples or test conditions.

4. ROQ

Calculates the difference in oxygen saturation between any two selected PO₂ values, revealing changes in hemoglobin oxygenation across a defined PO₂ range.

5. Multi-Curve Comparison

Display multiple test curves simultaneously to compare:

Leftward / rightward curve shifts
Changes in P50
Changes in oxygen affinity
Oxygen-binding characteristics
Oxygen-release characteristics
Differences between test groups

5. Applications

1. Red Blood Cell Oxygen-Transport Research

Assessment of oxygen binding and release in whole blood
Red blood cell oxygen-affinity studies
Studies of changes in red blood cell function

2. Blood Storage Research

Changes in red blood cell function during storage
Effects of storage conditions on oxygen binding and release
Effects of processing methods on hemoglobin oxygen affinity

3. Hypoxia and High-Altitude Research

Changes in hemoglobin oxygen affinity at high altitude
Mechanisms of adaptation to hypoxia
High-altitude-related changes in red blood cell function

4. Fundamental Blood Research

Supports evaluation of hemoglobin oxygen-transport function in research related to erythrocytosis, hyperviscosity, hemolysis, thrombosis, and other blood conditions.

5. Hemoglobin and Oxygen-Carrier Research

Hemoglobin functional assessment
Evaluation of hemoglobin-based oxygen carriers
Effects of formulation and processing conditions on oxygen binding and release

6. Software

BL10 Data Acquisition Software

For experiment monitoring, parameter display, and raw-data acquisition.

BL10 Viewer Data Analysis Software

For curve processing, parameter analysis, and comparison of test results.

7. System Components

Bloodox-2019 includes the following modules:

Dual-wavelength optical detection system
Clark oxygen electrode
Sample chamber
Gas-flow control system
Temperature-control system
Temperature sensor
Magnetic stirring system
Electronic control module
Touchscreen control system
Data acquisition and analysis software

8. Technical Specifications

Item	Specification
PO₂ measurement	Clark oxygen electrode
Hemoglobin measurement	Dual-wavelength spectrophotometry
Gases	Air / nitrogen
Gas-flow setting range	0–20 mL/min
Measurement temperature	37 ± 0.1 ℃
Sample requirement	Approx. 3–6 mg hemoglobin
Communication interface	Serial-to-USB
Power supply	AC 110–240 V，50/60 Hz
Main unit dimensions	64 × 40 × 32 cm
Data acquisition software	BL10
Data analysis software	BL10 Viewer

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